Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4580995..4581805 Replicon chromosome
Accession NZ_CP089882
Organism Klebsiella pneumoniae strain KP059

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LVQ69_RS22555 Protein ID WP_023279404.1
Coordinates 4580995..4581528 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVQ69_RS22560 Protein ID WP_002887278.1
Coordinates 4581539..4581805 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ69_RS22550 (LVQ69_22520) 4579826..4580947 + 1122 WP_002887282.1 cupin domain-containing protein -
LVQ69_RS22555 (LVQ69_22525) 4580995..4581528 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LVQ69_RS22560 (LVQ69_22530) 4581539..4581805 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVQ69_RS22565 (LVQ69_22535) 4581908..4583341 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LVQ69_RS22570 (LVQ69_22540) 4583331..4584014 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LVQ69_RS22575 (LVQ69_22545) 4584186..4585571 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LVQ69_RS22580 (LVQ69_22550) 4585589..4585933 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T229587 WP_023279404.1 NZ_CP089882:c4581528-4580995 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229587 NZ_CP124495:2939337-2939615 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229587 WP_002887278.1 NZ_CP089882:c4581805-4581539 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229587 NZ_CP124495:2939144-2939335 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References