Detailed information of TA system TA229570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5065653..5066278
• Replicon: chromosome
• Accession: NZ_CP089880
• Organism: Klebsiella pneumoniae strain KP309

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVQ72_RS24705
• Protein ID: WP_021312635.1
• Coordinates: 5065653..5066036 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: LVQ72_RS24710
• Protein ID: WP_004150355.1
• Coordinates: 5066036..5066278 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ72_RS24690 (LVQ72_24655)	5063019..5063921	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
LVQ72_RS24695 (LVQ72_24660)	5063918..5064553	+	636	WP_048255596.1	formate dehydrogenase cytochrome b556 subunit	-
LVQ72_RS24700 (LVQ72_24665)	5064550..5065479	+	930	WP_004146237.1	formate dehydrogenase accessory protein FdhE	-
LVQ72_RS24705 (LVQ72_24670)	5065653..5066036	-	384	WP_021312635.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVQ72_RS24710 (LVQ72_24675)	5066036..5066278	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
LVQ72_RS24715 (LVQ72_24680)	5066483..5067400	+	918	WP_032412655.1	alpha/beta hydrolase	-
LVQ72_RS24720 (LVQ72_24685)	5067415..5068356	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
LVQ72_RS24725 (LVQ72_24690)	5068401..5068838	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
LVQ72_RS24730 (LVQ72_24695)	5068835..5069695	-	861	WP_004146232.1	virulence factor BrkB family protein	-
LVQ72_RS24735 (LVQ72_24700)	5069689..5070288	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14349.56 Da
• Isoelectric Point: 7.3178

>T229570 WP_021312635.1 NZ_CP089880:c5066036-5065653 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVATPYEIHPE

Nucleotide

• Length: 384 bp

>T229570 NZ_CP124492:c4076280-4075993 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT229570 WP_004150355.1 NZ_CP089880:c5066278-5066036 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT229570 NZ_CP124492:c4076006-4075704 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9