Detailed information of TA system TA022957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1304662..1305308
• Replicon: chromosome
• Accession: NC_011001
• Organism: Burkholderia cenocepacia J2315

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B4EGU9
• Locus tag: QU43_RS60325
• Protein ID: WP_006491144.1
• Coordinates: 1304901..1305308 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4EGU8
• Locus tag: QU43_RS60320
• Protein ID: WP_006491146.1
• Coordinates: 1304662..1304904 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QU43_RS60305	1300657..1302063	+	1407	WP_006491136.1	aspartate ammonia-lyase	-
QU43_RS60310	1302141..1303646	+	1506	WP_006491141.1	amino acid permease	-
QU43_RS60315	1303720..1304403	-	684	WP_006491145.1	phospholipase	-
QU43_RS60320	1304662..1304904	+	243	WP_006491146.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
QU43_RS60325	1304901..1305308	+	408	WP_006491144.1	type II toxin-antitoxin system VapC family toxin	Toxin
QU43_RS76190	1305425..1305652	-	228	WP_006491154.1	hypothetical protein	-
QU43_RS60330	1305710..1308085	+	2376	WP_006491138.1	MCP four helix bundle domain-containing protein	-
QU43_RS60335	1308108..1308836	+	729	WP_034178465.1	chemotaxis protein	-
QU43_RS60340	1308833..1309414	+	582	WP_006486232.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14822.93 Da
• Isoelectric Point: 6.2165

>T22957 WP_006491144.1 NC_011001:1304901-1305308 [Burkholderia cenocepacia J2315]
MKFLLDTNAVIAILKGEPAILARLHAWHPADFGIPAIVAHALYYGAYKSQRAAANVARVEALQFEVVSFDTEDAQHAGEI
RAHLIAAGTPIGPYDALIAGQARARQLVLVTHNVREFERVPRLQFEDWLAEPSAD

Nucleotide

• Length: 408 bp

>T22957 NC_011001:1304901-1305308 [Burkholderia cenocepacia J2315]
ATGAAGTTCCTGCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGGATCCCCGCGATCGTCGCGCACGCGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCGG
CGAACGTCGCGCGTGTCGAGGCGTTGCAGTTCGAGGTCGTATCATTCGACACCGAGGATGCACAGCATGCGGGTGAAATT
CGCGCGCACCTGATCGCGGCCGGCACGCCGATTGGTCCATACGACGCCTTGATCGCAGGGCAGGCGCGCGCGCGGCAACT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTAGCCGAACCGAGCG
CAGACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.02 Da
• Isoelectric Point: 5.0197

>AT22957 WP_006491146.1 NC_011001:1304662-1304904 [Burkholderia cenocepacia J2315]
MDTAKLFKHGGSQAVRLPKDFRFDTAEVRIRRHGAAVILEPMPQDWAWLAPLIGPVDADFEAAATTQPSAQERSGLDVFE

Nucleotide

• Length: 243 bp

>AT22957 NC_011001:1304662-1304904 [Burkholderia cenocepacia J2315]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTGCCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCCGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA

Structures

Toxin

• AlphaFold DB: B4EGU9

Antitoxin

• AlphaFold DB: B4EGU8