Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4466940..4467750 Replicon chromosome
Accession NZ_CP089880
Organism Klebsiella pneumoniae strain KP309

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LVQ72_RS21845 Protein ID WP_004178461.1
Coordinates 4466940..4467473 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVQ72_RS21850 Protein ID WP_002887278.1
Coordinates 4467484..4467750 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ72_RS21840 (LVQ72_21810) 4465771..4466892 + 1122 WP_004186850.1 cupin domain-containing protein -
LVQ72_RS21845 (LVQ72_21815) 4466940..4467473 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LVQ72_RS21850 (LVQ72_21820) 4467484..4467750 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVQ72_RS21855 (LVQ72_21825) 4467853..4469286 - 1434 WP_048255468.1 Cu(+)/Ag(+) sensor histidine kinase -
LVQ72_RS21860 (LVQ72_21830) 4469276..4469959 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
LVQ72_RS21865 (LVQ72_21835) 4470131..4471516 + 1386 WP_048255467.1 efflux transporter outer membrane subunit -
LVQ72_RS21870 (LVQ72_21840) 4471534..4471878 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T229565 WP_004178461.1 NZ_CP089880:c4467473-4466940 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229565 NZ_CP124492:2699372-2699479 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229565 WP_002887278.1 NZ_CP089880:c4467750-4467484 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229565 NZ_CP124492:c2699315-2699260 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References