Detailed information of TA system TA229550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 169621..170531
• Replicon: plasmid pKP308-2
• Accession: NZ_CP089879
• Organism: Klebsiella pneumoniae strain KP308

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LVQ70_RS28325
• Protein ID: WP_032413375.1
• Coordinates: 169621..170091 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: LVQ70_RS28330
• Protein ID: WP_032413443.1
• Coordinates: 170088..170531 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ70_RS28305 (LVQ70_28275)	165232..166965	+	1734	WP_004118832.1	ABC transporter ATP-binding protein	-
LVQ70_RS28310 (LVQ70_28280)	167200..168168	-	969	WP_162887377.1	IS5 family transposase	-
LVQ70_RS28315 (LVQ70_28285)	168302..168679	+	378	Protein_172	integrase core domain-containing protein	-
LVQ70_RS28320 (LVQ70_28290)	169251..169511	+	261	WP_017901409.1	hypothetical protein	-
LVQ70_RS28325 (LVQ70_28295)	169621..170091	-	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
LVQ70_RS28330 (LVQ70_28300)	170088..170531	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
LVQ70_RS28335 (LVQ70_28305)	170631..171143	-	513	Protein_176	DUF4113 domain-containing protein	-
LVQ70_RS28340 (LVQ70_28310)	171244..171429	-	186	WP_071571073.1	DUF2188 domain-containing protein	-
LVQ70_RS28345 (LVQ70_28315)	171449..171673	+	225	Protein_178	transposase	-
LVQ70_RS28350 (LVQ70_28320)	171807..171932	-	126	Protein_179	transposase	-
LVQ70_RS28355 (LVQ70_28325)	171990..172337	-	348	WP_032413377.1	hypothetical protein	-
LVQ70_RS28360 (LVQ70_28330)	172474..173652	-	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
LVQ70_RS28365 (LVQ70_28335)	173699..174697	-	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(4)-Ia / aac(3)-IVa / dfrA12 / aadA2 / aph(3')-Ia / tet(A)	-	1..216726	216726
	-	inside	IScluster/Tn	-	-	167200..171962	4762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T229550 WP_032413375.1 NZ_CP089879:c170091-169621 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T229550 NZ_CP124490:c4018410-4018123 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT229550 WP_032413443.1 NZ_CP089879:c170531-170088 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT229550 NZ_CP124490:c4018136-4017834 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4