Detailed information of TA system TA022955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3504973..3505505
• Replicon: chromosome
• Accession: NC_011000
• Organism: Burkholderia cenocepacia J2315

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: QU43_RS52780
• Protein ID: WP_006484901.1
• Coordinates: 3505182..3505505 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: QU43_RS74140
• Protein ID: WP_012492929.1
• Coordinates: 3504973..3505185 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QU43_RS52745	3500316..3500828	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
QU43_RS52750	3500848..3501597	+	750	WP_006484965.1	tol-pal system protein YbgF	-
QU43_RS52760	3502025..3502345	-	321	WP_012492928.1	helix-turn-helix domain-containing protein	-
QU43_RS52765	3502484..3502879	+	396	WP_006484952.1	hypothetical protein	-
QU43_RS52770	3502947..3503399	+	453	WP_006484947.1	hypothetical protein	-
QU43_RS52775	3503667..3504686	+	1020	WP_012492245.1	IS110-like element ISBcen5 family transposase	-
QU43_RS74140	3504973..3505185	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
QU43_RS52780	3505182..3505505	+	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QU43_RS52785	3505825..3506349	+	525	WP_012492930.1	hypothetical protein	-
QU43_RS74145	3506351..3507376	+	1026	WP_012492931.1	relaxase/mobilization nuclease domain-containing protein	-
QU43_RS52790	3507499..3507942	-	444	WP_011544671.1	YiaA/YiaB family protein	-
QU43_RS52795	3508046..3508993	-	948	WP_006484950.1	AraC family transcriptional regulator	-
QU43_RS52800	3509123..3509452	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
QU43_RS52810	3509653..3510486	+	834	WP_012492932.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3502025..3511248	9223
	-	flank	IS/Tn	-	-	3503667..3504686	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T22955 WP_006484901.1 NC_011000:3505182-3505505 [Burkholderia cenocepacia J2315]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T22955 NC_011000:3505182-3505505 [Burkholderia cenocepacia J2315]
ATGAATCGCGGGAGCATTGTCACAGTCGCGCTCCAAGGTGACTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGCGTAACGTTGCTGCTAATGTCGAGCGCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT22955 WP_012492929.1 NC_011000:3504973-3505185 [Burkholderia cenocepacia J2315]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT22955 NC_011000:3504973-3505185 [Burkholderia cenocepacia J2315]
ATGGCAGCTACCACTTCCGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGTCCAATTCAGATATG
GGTGCCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGTTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7