Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5187002..5187627 Replicon chromosome
Accession NZ_CP089877
Organism Klebsiella pneumoniae strain KP308

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVQ70_RS25345 Protein ID WP_002882817.1
Coordinates 5187002..5187385 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVQ70_RS25350 Protein ID WP_004150355.1
Coordinates 5187385..5187627 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ70_RS25330 (LVQ70_25300) 5184368..5185270 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LVQ70_RS25335 (LVQ70_25305) 5185267..5185902 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVQ70_RS25340 (LVQ70_25310) 5185899..5186828 + 930 WP_025368581.1 formate dehydrogenase accessory protein FdhE -
LVQ70_RS25345 (LVQ70_25315) 5187002..5187385 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVQ70_RS25350 (LVQ70_25320) 5187385..5187627 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVQ70_RS25355 (LVQ70_25325) 5187832..5188749 + 918 WP_025368582.1 alpha/beta hydrolase -
LVQ70_RS25360 (LVQ70_25330) 5188764..5189705 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVQ70_RS25365 (LVQ70_25335) 5189750..5190187 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVQ70_RS25370 (LVQ70_25340) 5190184..5191044 - 861 WP_004146232.1 virulence factor BrkB family protein -
LVQ70_RS25375 (LVQ70_25345) 5191038..5191637 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229548 WP_002882817.1 NZ_CP089877:c5187385-5187002 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229548 NZ_CP124490:c3908441-3908127 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229548 WP_004150355.1 NZ_CP089877:c5187627-5187385 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229548 NZ_CP124490:c3908677-3908444 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References