Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3997848..3998467 Replicon chromosome
Accession NZ_CP089877
Organism Klebsiella pneumoniae strain KP308

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LVQ70_RS19725 Protein ID WP_002892050.1
Coordinates 3998249..3998467 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LVQ70_RS19720 Protein ID WP_002892066.1
Coordinates 3997848..3998222 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ70_RS19710 (LVQ70_19690) 3993000..3994193 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LVQ70_RS19715 (LVQ70_19695) 3994216..3997362 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LVQ70_RS19720 (LVQ70_19700) 3997848..3998222 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LVQ70_RS19725 (LVQ70_19705) 3998249..3998467 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LVQ70_RS19730 (LVQ70_19710) 3998626..3999192 + 567 WP_002892030.1 maltose O-acetyltransferase -
LVQ70_RS19735 (LVQ70_19715) 3999164..3999304 - 141 WP_004147370.1 hypothetical protein -
LVQ70_RS19740 (LVQ70_19720) 3999325..3999795 + 471 WP_002892026.1 YlaC family protein -
LVQ70_RS19745 (LVQ70_19725) 3999770..4001221 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LVQ70_RS19750 (LVQ70_19730) 4001322..4002020 + 699 WP_004177238.1 GNAT family protein -
LVQ70_RS19755 (LVQ70_19735) 4002017..4002157 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LVQ70_RS19760 (LVQ70_19740) 4002157..4002420 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T229544 WP_002892050.1 NZ_CP089877:3998249-3998467 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T229544 NZ_CP124490:2692481-2692588 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT229544 WP_002892066.1 NZ_CP089877:3997848-3998222 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT229544 NZ_CP124490:c2692424-2692369 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References