Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2575520..2577108 Replicon chromosome
Accession NZ_CP089877
Organism Klebsiella pneumoniae strain KP308

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag LVQ70_RS12625 Protein ID WP_002904792.1
Coordinates 2575520..2576842 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag LVQ70_RS12630 Protein ID WP_002904790.1
Coordinates 2576842..2577108 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ70_RS12610 (LVQ70_12600) 2570576..2573077 + 2502 WP_004151634.1 fimbria/pilus outer membrane usher protein -
LVQ70_RS12615 (LVQ70_12605) 2573065..2574069 + 1005 WP_002904798.1 fimbrial protein -
LVQ70_RS12620 (LVQ70_12610) 2574211..2575503 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
LVQ70_RS12625 (LVQ70_12615) 2575520..2576842 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
LVQ70_RS12630 (LVQ70_12620) 2576842..2577108 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LVQ70_RS12635 (LVQ70_12625) 2577374..2577700 - 327 WP_002904788.1 hydroxyisourate hydrolase -
LVQ70_RS12640 (LVQ70_12630) 2577697..2578197 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LVQ70_RS12645 (LVQ70_12635) 2578175..2579554 - 1380 WP_002904785.1 nucleobase:cation symporter-2 family protein -
LVQ70_RS12650 (LVQ70_12640) 2579900..2581054 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
LVQ70_RS12655 (LVQ70_12645) 2581035..2581958 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T229543 WP_002904792.1 NZ_CP089877:c2576842-2575520 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T229543 NZ_CP124490:2162088-2162190 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT229543 WP_002904790.1 NZ_CP089877:c2577108-2576842 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT229543 NZ_CP124490:c2162228-2161960 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References