Detailed information of TA system TA229530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55994..56519
• Replicon: plasmid pKP106-2
• Accession: NZ_CP089876
• Organism: Klebsiella pneumoniae strain KP106

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LVQ73_RS26640
• Protein ID: WP_013023785.1
• Coordinates: 56214..56519 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LVQ73_RS26635
• Protein ID: WP_001568025.1
• Coordinates: 55994..56212 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ73_RS26615 (LVQ73_26590)	51150..51929	+	780	WP_013023780.1	hypothetical protein	-
LVQ73_RS26620 (LVQ73_26595)	52136..53728	+	1593	WP_015344964.1	hypothetical protein	-
LVQ73_RS26625 (LVQ73_26600)	53762..54889	+	1128	WP_013023782.1	DUF4238 domain-containing protein	-
LVQ73_RS26630 (LVQ73_26605)	54886..55176	+	291	WP_013023783.1	hypothetical protein	-
LVQ73_RS26635 (LVQ73_26610)	55994..56212	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LVQ73_RS26640 (LVQ73_26615)	56214..56519	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LVQ73_RS26645 (LVQ73_26620)	56688..57083	+	396	WP_017899885.1	hypothetical protein	-
LVQ73_RS26650 (LVQ73_26625)	57110..57424	+	315	WP_053389906.1	hypothetical protein	-
LVQ73_RS26655 (LVQ73_26630)	57435..58451	+	1017	WP_017899884.1	hypothetical protein	-
LVQ73_RS26660 (LVQ73_26635)	58649..59443	+	795	WP_004197635.1	site-specific integrase	-
LVQ73_RS26665 (LVQ73_26640)	59899..60201	-	303	WP_004197636.1	hypothetical protein	-
LVQ73_RS26670 (LVQ73_26645)	60198..60824	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-3 / blaTEM-1B / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2	-	1..110438	110438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T229530 WP_013023785.1 NZ_CP089876:56214-56519 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T229530 NZ_CP124487:c4228338-4227961 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT229530 WP_001568025.1 NZ_CP089876:55994-56212 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT229530 NZ_CP124487:c4228795-4228427 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8