Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 156849..157759 Replicon plasmid pKP106-1
Accession NZ_CP089875
Organism Klebsiella pneumoniae strain KP106

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LVQ73_RS26180 Protein ID WP_004144067.1
Coordinates 156849..157319 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag LVQ73_RS26185 Protein ID WP_023287184.1
Coordinates 157316..157759 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ73_RS26160 (LVQ73_26135) 152547..153515 - 969 WP_167813129.1 IS5 family transposase -
LVQ73_RS26165 (LVQ73_26140) 153506..153787 + 282 Protein_158 IS481 family transposase -
LVQ73_RS26175 (LVQ73_26150) 155794..156762 - 969 WP_000654811.1 IS5 family transposase -
LVQ73_RS26180 (LVQ73_26155) 156849..157319 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LVQ73_RS26185 (LVQ73_26160) 157316..157759 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
LVQ73_RS26190 (LVQ73_26165) 157860..159002 - 1143 Protein_163 translesion error-prone DNA polymerase V subunit UmuC -
LVQ73_RS26195 (LVQ73_26170) 159055..161283 - 2229 WP_023287182.1 ThiF family adenylyltransferase -
LVQ73_RS26200 (LVQ73_26175) 161280..162461 - 1182 WP_023287181.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib cheD 1..186670 186670


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T229529 WP_004144067.1 NZ_CP089875:c157319-156849 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T229529 NZ_CP124487:c4157681-4157394 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT229529 WP_023287184.1 NZ_CP089875:c157759-157316 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT229529 NZ_CP124487:c4157407-4157105 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References