Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4542476..4543286 Replicon chromosome
Accession NZ_CP089874
Organism Klebsiella pneumoniae strain KP106

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LVQ73_RS22325 Protein ID WP_004178461.1
Coordinates 4542476..4543009 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LVQ73_RS22330 Protein ID WP_002887278.1
Coordinates 4543020..4543286 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ73_RS22320 (4541307) 4541307..4542428 + 1122 WP_277722162.1 cupin domain-containing protein -
LVQ73_RS22325 (4542476) 4542476..4543009 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LVQ73_RS22330 (4543020) 4543020..4543286 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LVQ73_RS22335 (4543389) 4543389..4544822 - 1434 WP_277722164.1 Cu(+)/Ag(+) sensor histidine kinase -
LVQ73_RS22340 (4544812) 4544812..4545495 - 684 WP_032105399.1 copper response regulator transcription factor CusR -
LVQ73_RS22345 (4545668) 4545668..4547053 + 1386 WP_277722401.1 efflux transporter outer membrane subunit -
LVQ73_RS22350 (4547071) 4547071..4547415 + 345 WP_048999680.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T229524 WP_004178461.1 NZ_CP089874:c4543009-4542476 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229524 NZ_CP124487:2942165-2942443 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229524 WP_002887278.1 NZ_CP089874:c4543286-4543020 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229524 NZ_CP124487:2941972-2942163 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References