Detailed information of TA system TA229509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 171025..171668
• Replicon: plasmid pKP037-1
• Accession: NZ_CP089871
• Organism: Klebsiella pneumoniae strain KP037

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LVQ68_RS26460
• Protein ID: WP_016236302.1
• Coordinates: 171025..171441 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LVQ68_RS26465
• Protein ID: WP_001261282.1
• Coordinates: 171438..171668 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ68_RS26440 (LVQ68_26400)	166393..166743	+	351	WP_004187110.1	DUF305 domain-containing protein	-
LVQ68_RS26445 (LVQ68_26405)	166929..167837	-	909	WP_032439686.1	HNH endonuclease	-
LVQ68_RS26450 (LVQ68_26410)	168382..169404	-	1023	WP_032439672.1	helicase UvrD	-
LVQ68_RS26455 (LVQ68_26415)	169389..170951	-	1563	WP_004206609.1	AAA family ATPase	-
LVQ68_RS26460 (LVQ68_26420)	171025..171441	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVQ68_RS26465 (LVQ68_26425)	171438..171668	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVQ68_RS26470 (LVQ68_26430)	171625..172047	+	423	WP_050485703.1	hypothetical protein	-
LVQ68_RS26475 (LVQ68_26435)	172247..173191	+	945	WP_032448293.1	hypothetical protein	-
LVQ68_RS26480 (LVQ68_26440)	173300..173692	+	393	WP_074422814.1	hypothetical protein	-
LVQ68_RS26485 (LVQ68_26445)	173750..174271	+	522	WP_032448294.1	hypothetical protein	-
LVQ68_RS26490 (LVQ68_26450)	174317..175102	+	786	WP_050485696.1	site-specific integrase	-
LVQ68_RS26495 (LVQ68_26455)	175484..176659	-	1176	Protein_186	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	iucA / iucB / iucC / iucD / iutA	1..231344	231344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T229509 WP_016236302.1 NZ_CP089871:c171441-171025 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T229509 NZ_CP124484:c132736-132431 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT229509 WP_001261282.1 NZ_CP089871:c171668-171438 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT229509 NZ_CP124484:c132956-132738 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3