Detailed information of TA system TA229508

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 126024..126760
• Replicon: plasmid pKP037-1
• Accession: NZ_CP089871
• Organism: Klebsiella pneumoniae strain KP037

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LVQ68_RS26240
• Protein ID: WP_004187044.1
• Coordinates: 126024..126506 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LVQ68_RS26245
• Protein ID: WP_003026799.1
• Coordinates: 126494..126760 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVQ68_RS26210 (LVQ68_26170)	121110..121259	+	150	Protein_129	transposase	-
LVQ68_RS26215 (LVQ68_26175)	121275..121385	+	111	Protein_130	IS3 family transposase	-
LVQ68_RS26220 (LVQ68_26180)	121527..121931	-	405	WP_277696269.1	helix-turn-helix domain-containing protein	-
LVQ68_RS26225 (LVQ68_26185)	121980..123326	-	1347	WP_077254728.1	ISNCY family transposase	-
LVQ68_RS26230 (LVQ68_26190)	123603..124307	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
LVQ68_RS26235 (LVQ68_26195)	124467..125813	-	1347	WP_077251107.1	ISNCY family transposase	-
LVQ68_RS26240 (LVQ68_26200)	126024..126506	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LVQ68_RS26245 (LVQ68_26205)	126494..126760	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LVQ68_RS26250 (LVQ68_26210)	127872..128681	-	810	WP_023329017.1	N-formylglutamate deformylase	-
LVQ68_RS26255 (LVQ68_26215)	128674..129882	-	1209	WP_032448288.1	imidazolonepropionase	-
LVQ68_RS26260 (LVQ68_26220)	129894..131288	-	1395	WP_032439652.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3'')-Ib / aph(6)-Id / tet(A)	iucA / iucB / iucC / iucD / iutA	1..231344	231344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T229508 WP_004187044.1 NZ_CP089871:c126506-126024 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T229508 NZ_CP124483:c4369743-4369570 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT229508 WP_003026799.1 NZ_CP089871:c126760-126494 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT229508 NZ_CP124483:c4370067-4369771 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66