Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5198087..5198712 Replicon chromosome
Accession NZ_CP089870
Organism Klebsiella pneumoniae strain KP037

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LVQ68_RS25200 Protein ID WP_002882817.1
Coordinates 5198087..5198470 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LVQ68_RS25205 Protein ID WP_004150355.1
Coordinates 5198470..5198712 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ68_RS25185 (5195453) 5195453..5196355 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LVQ68_RS25190 (5196352) 5196352..5196987 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LVQ68_RS25195 (5196984) 5196984..5197913 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LVQ68_RS25200 (5198087) 5198087..5198470 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LVQ68_RS25205 (5198470) 5198470..5198712 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LVQ68_RS25210 (5198917) 5198917..5199834 + 918 WP_032422045.1 alpha/beta hydrolase -
LVQ68_RS25215 (5199848) 5199848..5200789 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LVQ68_RS25220 (5200834) 5200834..5201271 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LVQ68_RS25225 (5201268) 5201268..5202128 - 861 WP_004146232.1 virulence factor BrkB family protein -
LVQ68_RS25230 (5202122) 5202122..5202721 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229507 WP_002882817.1 NZ_CP089870:c5198470-5198087 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229507 NZ_CP124483:c4159018-4158641 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229507 WP_004150355.1 NZ_CP089870:c5198712-5198470 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229507 NZ_CP124483:c4159475-4159107 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References