Detailed information of TA system TA022950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 127386..127966
• Replicon: plasmid pA
• Accession: NC_010998
• Organism: Rhizobium etli CIAT 652

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B3Q1A1
• Locus tag: RHECIAT_RS22255
• Protein ID: WP_012490497.1
• Coordinates: 127386..127769 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RHECIAT_RS22260
• Protein ID: WP_041683976.1
• Coordinates: 127766..127966 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHECIAT_RS22240	124408..124626	-	219	WP_012490494.1	hypothetical protein	-
RHECIAT_RS22245	124787..125072	+	286	Protein_111	tail-specific protease	-
RHECIAT_RS22250	125345..127306	+	1962	WP_012490496.1	elongation factor G	-
RHECIAT_RS22255	127386..127769	-	384	WP_012490497.1	type II toxin-antitoxin system VapC family toxin	Toxin
RHECIAT_RS22260	127766..127966	-	201	WP_041683976.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RHECIAT_RS22265	128301..130418	+	2118	WP_012490498.1	carboxy terminal-processing peptidase	-
RHECIAT_RS22270	130571..130816	+	246	WP_049772079.1	DUF1778 domain-containing protein	-
RHECIAT_RS22275	130806..131305	+	500	Protein_117	GNAT family N-acetyltransferase	-
RHECIAT_RS22280	131434..132330	+	897	WP_012490501.1	GGDEF domain-containing protein	-
RHECIAT_RS22285	132354..132695	-	342	WP_041683978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..414090	414090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14246.40 Da
• Isoelectric Point: 7.5223

>T22950 WP_012490497.1 NC_010998:c127769-127386 [Rhizobium etli CIAT 652]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGRLRDRSNVIAFLAAQRQALVATHDEVMMMIDRHAIFSM
GIGYTDAHLIASVLLDRRAALWTRDKRLRAAAEKAGASLHTPDNARS

Nucleotide

• Length: 384 bp

>T22950 NC_010998:c127769-127386 [Rhizobium etli CIAT 652]
GTGATACTTGCCGACACGTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGGCTTCGGGATCGCAGTAACGTGATAGCTTTCC
TGGCGGCCCAGCGCCAAGCGTTGGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCATTTTCAGCATG
GGCATCGGCTACACCGATGCCCATTTGATCGCTTCAGTTCTACTCGATCGGCGAGCGGCCTTATGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTTCACACGCCCGACAATGCGCGAAGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7196.32 Da
• Isoelectric Point: 11.3760

>AT22950 WP_041683976.1 NC_010998:c127966-127766 [Rhizobium etli CIAT 652]
MRSTINLDDTLIERARFLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT22950 NC_010998:c127966-127766 [Rhizobium etli CIAT 652]
ATGCGATCAACCATCAATCTCGACGACACCCTCATTGAAAGGGCCAGGTTCCTAACCGGTACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA

Structures

Toxin

• AlphaFold DB: B3Q1A1