Detailed information of TA system TA229488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 10032..11140
• Replicon: plasmid pBNS11-360kb
• Accession: NZ_CP089851
• Organism: Ligilactobacillus salivarius strain BNS11

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LVD18_RS09330
• Protein ID: WP_000233000.1
• Coordinates: 10271..11140 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LVD18_RS09325
• Protein ID: WP_000205227.1
• Coordinates: 10032..10256 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVD18_RS09315 (LVD18_09315)	6074..7393	+	1320	WP_192044369.1	MATE family efflux transporter	-
LVD18_RS09320 (LVD18_09320)	7386..9692	+	2307	WP_192044368.1	bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB	-
LVD18_RS09325 (LVD18_09325)	10032..10256	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LVD18_RS09330 (LVD18_09330)	10271..11140	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LVD18_RS09335 (LVD18_09335)	11121..11855	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LVD18_RS09340 (LVD18_09340)	11888..12751	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LVD18_RS09345 (LVD18_09345)	13024..13248	+	225	Protein_12	GNAT family N-acetyltransferase	-
LVD18_RS09350 (LVD18_09350)	13249..14688	+	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
LVD18_RS09355 (LVD18_09355)	14765..14935	+	171	Protein_14	transposase	-
LVD18_RS09360 (LVD18_09360)	15235..15930	+	696	WP_232751521.1	FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T229488 WP_000233000.1 NZ_CP089851:10271-11140 [Ligilactobacillus salivarius]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T229488 NZ_CP124483:c46336-45959 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT229488 WP_000205227.1 NZ_CP089851:10032-10256 [Ligilactobacillus salivarius]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT229488 NZ_CP124483:c46794-46426 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: R3JIN1