Detailed information of TA system TA229483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1930925..1931511
• Replicon: chromosome
• Accession: NZ_CP089849
• Organism: Pseudomonas aeruginosa strain PA0523

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LVE67_RS09220
• Protein ID: WP_003120987.1
• Coordinates: 1930925..1931224 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LVE67_RS09225
• Protein ID: WP_003448662.1
• Coordinates: 1931221..1931511 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVE67_RS09200 (LVE67_09200)	1926038..1926571	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
LVE67_RS09205 (LVE67_09205)	1926561..1927886	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
LVE67_RS09210 (LVE67_09210)	1927890..1929599	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
LVE67_RS09215 (LVE67_09215)	1929599..1930723	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
LVE67_RS09220 (LVE67_09220)	1930925..1931224	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVE67_RS09225 (LVE67_09225)	1931221..1931511	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LVE67_RS09230 (LVE67_09230)	1931582..1931926	-	345	WP_016851612.1	hypothetical protein	-
LVE67_RS09235 (LVE67_09235)	1932067..1934043	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
LVE67_RS09240 (LVE67_09240)	1934040..1935929	-	1890	WP_016851610.1	hypothetical protein	-
LVE67_RS09245 (LVE67_09245)	1936151..1936360	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1850962..1961108	110146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T229483 WP_003120987.1 NZ_CP089849:1930925-1931224 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T229483 NZ_CP124481:c4345850-4345536 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT229483 WP_003448662.1 NZ_CP089849:1931221-1931511 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT229483 NZ_CP124481:c4345539-4345123 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G8CP73