Detailed information of TA system TA229481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1174544..1175130
• Replicon: chromosome
• Accession: NZ_CP089849
• Organism: Pseudomonas aeruginosa strain PA0523

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LVE67_RS05530
• Protein ID: WP_003120987.1
• Coordinates: 1174544..1174843 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LVE67_RS05535
• Protein ID: WP_003448662.1
• Coordinates: 1174840..1175130 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVE67_RS05510 (LVE67_05510)	1169660..1170193	-	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-
LVE67_RS05515 (LVE67_05515)	1170183..1171508	-	1326	WP_003120992.1	type 4b pilus protein PilO2	-
LVE67_RS05520 (LVE67_05520)	1171512..1173221	-	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
LVE67_RS05525 (LVE67_05525)	1173221..1174342	-	1122	WP_232772652.1	TcpQ domain-containing protein	-
LVE67_RS05530 (LVE67_05530)	1174544..1174843	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVE67_RS05535 (LVE67_05535)	1174840..1175130	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LVE67_RS05540 (LVE67_05540)	1175213..1175548	-	336	WP_022579623.1	hypothetical protein	-
LVE67_RS05545 (LVE67_05545)	1175687..1177663	-	1977	WP_023093671.1	DEAD/DEAH box helicase	-
LVE67_RS05550 (LVE67_05550)	1177660..1179555	-	1896	WP_155713823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1104675..1203554	98879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T229481 WP_003120987.1 NZ_CP089849:1174544-1174843 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T229481 NZ_CP124481:4166090-4166467 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT229481 WP_003448662.1 NZ_CP089849:1174840-1175130 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT229481 NZ_CP124481:4165633-4166013 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: G8CP73