Detailed information of TA system TA229479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 158781..159286
• Replicon: chromosome
• Accession: NZ_CP089849
• Organism: Pseudomonas aeruginosa strain PA0523

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: LVE67_RS00715
• Protein ID: WP_003083773.1
• Coordinates: 158781..159062 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: LVE67_RS00720
• Protein ID: WP_003083775.1
• Coordinates: 159059..159286 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVE67_RS00690 (LVE67_00690)	154032..155381	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
LVE67_RS00695 (LVE67_00695)	155430..156116	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
LVE67_RS00700 (LVE67_00700)	156217..156951	+	735	WP_003110658.1	GntR family transcriptional regulator	-
LVE67_RS00705 (LVE67_00705)	157131..157541	+	411	WP_003110659.1	aegerolysin family protein	-
LVE67_RS00710 (LVE67_00710)	157573..158481	-	909	WP_003083769.1	LysR family transcriptional regulator	-
LVE67_RS00715 (LVE67_00715)	158781..159062	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
LVE67_RS00720 (LVE67_00720)	159059..159286	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
LVE67_RS00725 (LVE67_00725)	159462..160082	-	621	WP_003101226.1	hypothetical protein	-
LVE67_RS00730 (LVE67_00730)	160183..160683	+	501	WP_003101228.1	LEA type 2 family protein	-
LVE67_RS00735 (LVE67_00735)	160756..161097	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
LVE67_RS00740 (LVE67_00740)	161179..162606	-	1428	WP_003083784.1	GABA permease	-
LVE67_RS00745 (LVE67_00745)	162775..164268	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T229479 WP_003083773.1 NZ_CP089849:c159062-158781 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T229479 NZ_CP124481:c3079914-3079663 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT229479 WP_003083775.1 NZ_CP089849:c159286-159059 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT229479 NZ_CP124481:c3079666-3079412 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9