Detailed information of TA system TA229478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3935083..3935648
• Replicon: chromosome
• Accession: NZ_CP089848
• Organism: Paralcaligenes sp. KSB-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LSG25_RS18030
• Protein ID: WP_232742255.1
• Coordinates: 3935083..3935454 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LSG25_RS18035
• Protein ID: WP_232742256.1
• Coordinates: 3935451..3935648 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG25_RS18005 (LSG25_18005)	3930173..3931144	+	972	WP_232742250.1	tripartite tricarboxylate transporter substrate binding protein	-
LSG25_RS18010 (LSG25_18010)	3931161..3932072	+	912	WP_232742251.1	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
LSG25_RS18015 (LSG25_18015)	3932099..3932896	-	798	WP_232742252.1	amidohydrolase family protein	-
LSG25_RS18020 (LSG25_18020)	3932920..3933855	-	936	WP_232742253.1	isocitrate lyase/PEP mutase family protein	-
LSG25_RS18025 (LSG25_18025)	3934139..3935059	+	921	WP_232742254.1	DMT family transporter	-
LSG25_RS18030 (LSG25_18030)	3935083..3935454	-	372	WP_232742255.1	type II toxin-antitoxin system VapC family toxin	Toxin
LSG25_RS18035 (LSG25_18035)	3935451..3935648	-	198	WP_232742256.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LSG25_RS18040 (LSG25_18040)	3935792..3939265	-	3474	WP_232742257.1	DUF748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13538.73 Da
• Isoelectric Point: 9.8865

>T229478 WP_232742255.1 NZ_CP089848:c3935454-3935083 [Paralcaligenes sp. KSB-10]
VILVDTSVWIDHLRKSDGELQTLLNAGQVLAHPFVIGELALGNLRQRDVVLDALQGLPQVSVATEEEVLRFIGQKTLYGI
GIGYIDAHLLAALRLSPGSRLWTRDRRLLAAATRLGLSIRRAH

Nucleotide

• Length: 372 bp

>T229478 NZ_CP124481:c3079666-3079412 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7111.23 Da
• Isoelectric Point: 6.2934

>AT229478 WP_232742256.1 NZ_CP089848:c3935648-3935451 [Paralcaligenes sp. KSB-10]
MRTTLALDDELLEKAQALTGTIEKTALVREALKALIQRESAKRLALLGGSEPLLDNVPRRQSEPA

Nucleotide

• Length: 198 bp

>AT229478 NZ_CP124481:c3079914-3079663 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA