Detailed information of TA system TA229477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3230032..3231087
• Replicon: chromosome
• Accession: NZ_CP089848
• Organism: Paralcaligenes sp. KSB-10

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LSG25_RS14910
• Protein ID: WP_232741693.1
• Coordinates: 3230509..3231087 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LSG25_RS14905
• Protein ID: WP_232741692.1
• Coordinates: 3230032..3230505 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG25_RS14880 (LSG25_14880)	3226272..3226649	+	378	Protein_2935	PAS domain S-box protein	-
LSG25_RS14885 (LSG25_14885)	3226638..3227171	+	534	WP_255696753.1	GGDEF domain-containing protein	-
LSG25_RS14890 (LSG25_14890)	3227223..3227987	+	765	WP_232741690.1	EAL domain-containing protein	-
LSG25_RS14895 (LSG25_14895)	3228387..3228827	+	441	WP_232741691.1	Hsp20/alpha crystallin family protein	-
LSG25_RS14900 (LSG25_14900)	3228937..3229257	+	321	WP_232744712.1	Hsp20/alpha crystallin family protein	-
LSG25_RS14905 (LSG25_14905)	3230032..3230505	+	474	WP_232741692.1	helix-turn-helix domain-containing protein	Antitoxin
LSG25_RS14910 (LSG25_14910)	3230509..3231087	+	579	WP_232741693.1	PIN domain-containing protein	Toxin
LSG25_RS14915 (LSG25_14915)	3231395..3232795	-	1401	WP_232741694.1	MFS transporter	-
LSG25_RS14920 (LSG25_14920)	3232950..3233882	+	933	WP_232741695.1	LysR family transcriptional regulator	-
LSG25_RS14925 (LSG25_14925)	3233965..3235824	-	1860	WP_232741696.1	sigma-54-dependent Fis family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-116)

Antitoxin

• Helix-turn-helix domain, group 17 (88-135)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21543.63 Da
• Isoelectric Point: 4.4911

>T229477 WP_232741693.1 NZ_CP089848:3230509-3231087 [Paralcaligenes sp. KSB-10]
MAGSYSYTVILDACVLYPAPLRDLLLSLAEADVFRGRWTSSIHDEWTRNVLAKRPDLKPDTLNRTVELMNEAVDDCLVEN
YEYLIDSLTLPDENDRHVLAAAIAGHADAIVTFNLKDFPSEIANAHGLEILHPDDFLVAQYDLAPVRMLKVVQTLRERLK
NPQRTAQELIATYLSQGLPQTCKLLEDAIGLI

Nucleotide

• Length: 579 bp

>T229477 NZ_CP124481:3066630-3067004 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17646.41 Da
• Isoelectric Point: 6.4917

>AT229477 WP_232741692.1 NZ_CP089848:3230032-3230505 [Paralcaligenes sp. KSB-10]
MSNDLDLDMVLDNKEDVELAKAAQRCIVSALDHSKAINIAIIEDGVERHDGSPLLRLPPKALRLIADLLGSLAQGKAVAI
IPKELDVTTQEAAMFLNVSRPFLVRLLEDGKIQYHKVGTHRRIRFEDVVKYKENRRKISEAALQKLTDQAQEMDMGY

Nucleotide

• Length: 474 bp

>AT229477 NZ_CP124481:3066173-3066541 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA