Detailed information of TA system TA022944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 4112602..4113181
• Replicon: chromosome
• Accession: NC_010995
• Organism: Cellvibrio japonicus Ueda107

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B3PFX8
• Locus tag: CJA_RS16550
• Protein ID: WP_012489007.1
• Coordinates: 4112602..4112895 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B3PFX9
• Locus tag: CJA_RS16555
• Protein ID: WP_012489008.1
• Coordinates: 4112897..4113181 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJA_RS16520	4108223..4109128	+	906	WP_041551687.1	DUF3473 domain-containing protein	-
CJA_RS16530	4109503..4110078	+	576	WP_041551688.1	hypothetical protein	-
CJA_RS16535	4110099..4110875	+	777	WP_012489005.1	DUF2974 domain-containing protein	-
CJA_RS16540	4110970..4111386	+	417	WP_041551690.1	hypothetical protein	-
CJA_RS19410	4111485..4111682	+	198	WP_148208914.1	hypothetical protein	-
CJA_RS16545	4111699..4112424	-	726	WP_012489006.1	phage antirepressor N-terminal domain-containing protein	-
CJA_RS16550	4112602..4112895	+	294	WP_012489007.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CJA_RS16555	4112897..4113181	+	285	WP_012489008.1	putative addiction module antidote protein	Antitoxin
CJA_RS19415	4113686..4113916	-	231	WP_148208915.1	hypothetical protein	-
CJA_RS16560	4114639..4115967	+	1329	WP_012489009.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
CJA_RS16565	4115998..4117068	+	1071	WP_012489010.1	dTDP-glucose 4,6-dehydratase	-
CJA_RS16570	4117091..4117966	+	876	WP_012489011.1	glucose-1-phosphate thymidylyltransferase RfbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbtL	4093736..4142198	48462

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10797.48 Da
• Isoelectric Point: 10.9498

>T22944 WP_012489007.1 NC_010995:4112602-4112895 [Cellvibrio japonicus Ueda107]
MNYLIQQTQTFAKWHKSLRDLRAKLAIARRIDRIKAGNLGDVKSVGEGVSELRIDVGAGYRVYFTVRNGVVVVLLAGGDK
STQQADIKRARQLAQEV

Nucleotide

• Length: 294 bp

>T22944 NC_010995:4112602-4112895 [Cellvibrio japonicus Ueda107]
ATGAACTACTTAATCCAGCAAACACAAACCTTCGCCAAGTGGCACAAGTCGTTACGTGATCTGCGCGCCAAGCTGGCGAT
TGCCCGCCGGATTGACCGGATTAAGGCTGGCAACCTGGGGGATGTGAAATCAGTGGGCGAAGGGGTATCTGAGTTGCGTA
TCGATGTGGGGGCAGGCTACCGCGTGTATTTTACGGTGCGCAACGGGGTTGTAGTGGTGTTGCTGGCCGGTGGCGATAAA
TCTACCCAGCAAGCCGACATTAAGCGAGCCAGGCAACTGGCGCAGGAGGTATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10097.58 Da
• Isoelectric Point: 4.5837

>AT22944 WP_012489008.1 NC_010995:4112897-4113181 [Cellvibrio japonicus Ueda107]
MAQTLIEFDLAELLDSEEAITEYLSQVLDDGDSDELLRAIGYIAKARGMAQIAAASGLGRESLYKALAPGAKPRFDTVLK
VIHALGIKLQAQHA

Nucleotide

• Length: 285 bp

>AT22944 NC_010995:4112897-4113181 [Cellvibrio japonicus Ueda107]
ATGGCACAGACATTAATCGAGTTTGACTTGGCTGAACTGCTGGACAGCGAGGAGGCCATCACCGAATACCTGTCTCAAGT
CCTGGACGATGGAGACAGCGACGAACTACTGCGCGCCATTGGCTATATCGCTAAGGCGCGAGGCATGGCACAGATTGCGG
CAGCTTCTGGCCTTGGACGCGAAAGCCTTTACAAGGCGCTGGCACCCGGCGCCAAGCCACGGTTTGATACGGTGTTAAAG
GTAATTCATGCCTTGGGTATTAAACTGCAAGCGCAACATGCGTAA

Structures

Toxin

• AlphaFold DB: B3PFX8

Antitoxin

• AlphaFold DB: B3PFX9