Detailed information of TA system TA229392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 299360..300151
• Replicon: chromosome
• Accession: NZ_CP089795
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00813

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C0Q119
• Locus tag: LUY22_RS01390
• Protein ID: WP_000369560.1
• Coordinates: 299633..300151 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q57IR1
• Locus tag: LUY22_RS01385
• Protein ID: WP_001275017.1
• Coordinates: 299360..299629 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY22_RS01365 (294941)	294941..295609	+	669	WP_000617729.1	cell division ATP-binding protein FtsE	-
LUY22_RS01370 (295602)	295602..296657	+	1056	WP_001081707.1	permease-like cell division protein FtsX	-
LUY22_RS01375 (296903)	296903..297757	+	855	WP_000159621.1	RNA polymerase sigma factor RpoH	-
LUY22_RS01380 (298079)	298079..299182	+	1104	WP_001533929.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LUY22_RS01385 (299360)	299360..299629	+	270	WP_001275017.1	DUF1778 domain-containing protein	Antitoxin
LUY22_RS01390 (299633)	299633..300151	+	519	WP_000369560.1	GNAT family N-acetyltransferase	Toxin
LUY22_RS01395 (300148)	300148..300531	-	384	WP_000778873.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LUY22_RS01400 (300953)	300953..302062	+	1110	WP_023224165.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LUY22_RS01405 (302122)	302122..303048	+	927	WP_000003005.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LUY22_RS01410 (303045)	303045..304322	+	1278	WP_000803766.1	branched chain amino acid ABC transporter permease LivM	-
LUY22_RS01415 (304319)	304319..305086	+	768	WP_000082083.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19414.16 Da
• Isoelectric Point: 8.9619

>T229392 WP_000369560.1 NZ_CP089795:299633-300151 [Salmonella enterica subsp. enterica serovar Kentucky]
VDNLTIEILADNAEYNWRQFDCGEASLNLFLTQHLQRQHNNKILRGYVLRTTTPERRVLGYYTLSGSCFERASLPSRTQQ
KRIPYQNIPSVTLGRLAVDLSLQGKGWGAILVTHAMKVVWSASLAVGIHGIFVEALNEKAQAFYQRLGFISLSGENEHAL
FYPTKSIEQLFG

Nucleotide

• Length: 519 bp

>T229392 NZ_CP124462:2222-2497 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10086.53 Da
• Isoelectric Point: 7.2056

>AT229392 WP_001275017.1 NZ_CP089795:299360-299629 [Salmonella enterica subsp. enterica serovar Kentucky]
MSALKKQRIDLRLTDGDKSMIEEAAAITNQSITQFMLNSAAERAAEVLEQHRRVILNEASWARVMDALSHPPTPNEKLKR
AAQRLQDME

Nucleotide

• Length: 270 bp

>AT229392 NZ_CP124462:1938-2222 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A5H5BA59

Antitoxin

• AlphaFold DB: A0A5I8URP5