Detailed information of TA system TA022936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2108912..2109627
• Replicon: chromosome
• Accession: NC_010939
• Organism: Actinobacillus pleuropneumoniae serovar 7 str. AP76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APP7_RS09995
• Protein ID: WP_005619039.1
• Coordinates: 2108912..2109340 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: APP7_RS10000
• Protein ID: WP_005599447.1
• Coordinates: 2109340..2109627 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APP7_RS09980	2104498..2106203	-	1706	Protein_1917	RelA/SpoT family protein	-
APP7_RS09985	2106299..2106580	-	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
APP7_RS09990	2106707..2108788	-	2082	WP_005618301.1	ATP-dependent DNA helicase RecG	-
APP7_RS09995	2108912..2109340	-	429	WP_005619039.1	type II toxin-antitoxin system VapC family toxin	Toxin
APP7_RS10000	2109340..2109627	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
APP7_RS10005	2109833..2111548	+	1716	WP_005618303.1	proline--tRNA ligase	-
APP7_RS10010	2111614..2111802	-	189	WP_005618305.1	hypothetical protein	-
APP7_RS10015	2111805..2112386	-	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
APP7_RS10020	2112475..2113431	+	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
APP7_RS10025	2113431..2114024	+	594	WP_005599453.1	sulfoxide reductase heme-binding subunit YedZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16508.97 Da
• Isoelectric Point: 8.9724

>T22936 WP_005619039.1 NC_010939:c2109340-2108912 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MFQYLFDTNIISELYKLGSNRMDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQA
KSFSINNEIALLASEYHIPNKMDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 429 bp

>T22936 NC_010939:c2109340-2108912 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGTTTCAATATCTTTTTGATACCAACATCATTTCCGAGCTTTATAAGCTAGGAAGCAATAGAATGGATACCAATGTACG
ACAATGGTTAGCAACGATTAAACCGAGTCAGACGAATATTAGCTGTATCACGCTTTCCGAAATAAAAACGGGGATTTTAC
TCAAAGCTCGTAAAGATCCTATTCAAGCCGAGCGGTTAAATCATTGGTTTACCCATAATGTATTAAGCGTTTATCAAGCT
AAATCCTTTTCTATAAATAATGAAATTGCGTTACTGGCTTCTGAATACCACATTCCAAATAAAATGGATTTAAACGATGC
TTATATTGCCGCAACCGCTAAATATCATAATTTAGTGCTTGTTACTCGTAATTTGAAAGACTTCAACCGTTGCGATATAC
GTTTATTTAATCCTTTCGAACCGAATTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT22936 WP_005599447.1 NC_010939:c2109627-2109340 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT22936 NC_010939:c2109627-2109340 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGCCAATTACTATTACCTCTCGAGAATTTAATCAACACGCCAGTAAAGTGCTGAAAATGGCAGAAAAAGAACCGGTTTT
TATCACTAAATGGGGAAAAATTGTCAGCGTTGTCTCCAATTATGTTGATTACAAACAGCACACAACGGTTGAGCCTAGCT
TTGAAGAACTATTTGCCTCATCTACGGATGAATCGGTTAGCGATGAGTTTTGGCAAGATTTTGAACAGACATTAACAAAA
ATTCGCAAAAGTAATAAATCCGAACAAATAGATTTTGGAGATGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5