Detailed information of TA system TA022935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1992759..1993387
• Replicon: chromosome
• Accession: NC_010939
• Organism: Actinobacillus pleuropneumoniae serovar 7 str. AP76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APP7_RS09300
• Protein ID: WP_005618201.1
• Coordinates: 1992989..1993387 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: APP7_RS09295
• Protein ID: WP_005613300.1
• Coordinates: 1992759..1992989 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APP7_RS09290	1990607..1992238	+	1632	WP_005618198.1	hypothetical protein	-
APP7_RS09295	1992759..1992989	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
APP7_RS09300	1992989..1993387	+	399	WP_005618201.1	tRNA(fMet)-specific endonuclease VapC	Toxin
APP7_RS09305	1993523..1993939	-	417	WP_005618204.1	DUF417 family protein	-
APP7_RS09310	1994185..1994835	+	651	WP_005618207.1	DUF2202 domain-containing protein	-
APP7_RS09315	1995015..1995794	-	780	WP_005602546.1	hypothetical protein	-
APP7_RS09320	1995867..1997189	-	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
APP7_RS09325	1997244..1997765	-	522	WP_005618211.1	ATP-dependent protease subunit HslV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(B)	-	1978077..2042160	64083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15124.41 Da
• Isoelectric Point: 6.9683

>T22935 WP_005618201.1 NC_010939:1992989-1993387 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYDAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKCGKILGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T22935 NC_010939:1992989-1993387 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGCTGACTTATATGCTTGATACTAATATTGCGATTTATGTGATTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACTCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGATGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACAATTCTTGATTATACGCATAAGGCGGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGTGCGGTAAGATTCTCGGCGAGAACGATATTCATATTGCCGCGCATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT22935 WP_005613300.1 NC_010939:1992759-1992989 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT22935 NC_010939:1992759-1992989 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGGAAGCATCAGTATTTATGACAAATCGTAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTAGTTGGTAATGAGCGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGAGTT
CGGCAACGGTAAGCGATGATTTTATGACAGAGCGTGAAATAAGCACTCAAGCTGAACGTGAGGAACTGTAA

Structures

Antitoxin

• AlphaFold DB: A3N328