Detailed information of TA system TA022934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1776744..1777475
• Replicon: chromosome
• Accession: NC_010939
• Organism: Actinobacillus pleuropneumoniae serovar 7 str. AP76

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B3GYM0
• Locus tag: APP7_RS08250
• Protein ID: WP_005618004.1
• Coordinates: 1776744..1777211 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: APP7_RS08255
• Protein ID: WP_005619269.1
• Coordinates: 1777215..1777475 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APP7_RS08225	1772102..1772473	-	372	WP_005598830.1	CrcB family protein	-
APP7_RS08230	1772476..1773540	-	1065	WP_012478549.1	MFS transporter	-
APP7_RS08235	1773635..1774744	+	1110	WP_005617998.1	anhydro-N-acetylmuramic acid kinase	-
APP7_RS08240	1774798..1775712	+	915	WP_005618000.1	N-acetylmuramic acid 6-phosphate etherase	-
APP7_RS08245	1775779..1776660	-	882	WP_012478550.1	50S ribosomal protein L11 methyltransferase	-
APP7_RS08250	1776744..1777211	-	468	WP_005618004.1	GNAT family N-acetyltransferase	Toxin
APP7_RS08255	1777215..1777475	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
APP7_RS08260	1777596..1779056	-	1461	WP_005618007.1	metalloprotease TldD	-
APP7_RS08265	1779186..1780445	+	1260	WP_005618010.1	tRNA lysidine(34) synthetase TilS	-
APP7_RS08270	1780472..1780762	-	291	WP_005602215.1	DUF5389 family protein	-
APP7_RS08275	1780764..1781657	-	894	WP_005618012.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (22-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17208.23 Da
• Isoelectric Point: 8.9312

>T22934 WP_005618004.1 NC_010939:c1777211-1776744 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MHALELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Nucleotide

• Length: 468 bp

>T22934 NC_010939:c1777211-1776744 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGCACGCACTGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGTGTGATGAAAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGTGCCTTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGTTCGGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGGTAAAAGCATTGCTTGTTCATGCGTTAAATCCGCAAGCAAAAC
AATTTTATTTAAAGTACGGTTTTTCATGTTCACCCATTGATGAGATGGTTTTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT22934 WP_005619269.1 NC_010939:c1777475-1777215 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT22934 NC_010939:c1777475-1777215 [Actinobacillus pleuropneumoniae serovar 7 str. AP76]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: B3GYM0

Antitoxin

• AlphaFold DB: A3N2I7