Detailed information of TA system TA229308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 299192..299983
• Replicon: chromosome
• Accession: NZ_CP089790
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00830

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C0Q119
• Locus tag: LUY12_RS01385
• Protein ID: WP_000369560.1
• Coordinates: 299465..299983 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q57IR1
• Locus tag: LUY12_RS01380
• Protein ID: WP_001275017.1
• Coordinates: 299192..299461 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY12_RS01360 (294773)	294773..295441	+	669	WP_000617729.1	cell division ATP-binding protein FtsE	-
LUY12_RS01365 (295434)	295434..296489	+	1056	WP_001081707.1	permease-like cell division protein FtsX	-
LUY12_RS01370 (296735)	296735..297589	+	855	WP_000159621.1	RNA polymerase sigma factor RpoH	-
LUY12_RS01375 (297911)	297911..299014	+	1104	WP_001533929.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LUY12_RS01380 (299192)	299192..299461	+	270	WP_001275017.1	DUF1778 domain-containing protein	Antitoxin
LUY12_RS01385 (299465)	299465..299983	+	519	WP_000369560.1	GNAT family N-acetyltransferase	Toxin
LUY12_RS01390 (299980)	299980..300363	-	384	WP_000778873.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LUY12_RS01395 (300785)	300785..301894	+	1110	WP_023224165.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LUY12_RS01400 (301954)	301954..302880	+	927	WP_000003005.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LUY12_RS01405 (302877)	302877..304154	+	1278	WP_000803766.1	branched chain amino acid ABC transporter permease LivM	-
LUY12_RS01410 (304151)	304151..304918	+	768	WP_000082083.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19414.16 Da
• Isoelectric Point: 8.9619

>T229308 WP_000369560.1 NZ_CP089790:299465-299983 [Salmonella enterica subsp. enterica serovar Kentucky]
VDNLTIEILADNAEYNWRQFDCGEASLNLFLTQHLQRQHNNKILRGYVLRTTTPERRVLGYYTLSGSCFERASLPSRTQQ
KRIPYQNIPSVTLGRLAVDLSLQGKGWGAILVTHAMKVVWSASLAVGIHGIFVEALNEKAQAFYQRLGFISLSGENEHAL
FYPTKSIEQLFG

Nucleotide

• Length: 519 bp

>T229308 NZ_CP124436:3484651-3485001 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10086.53 Da
• Isoelectric Point: 7.2056

>AT229308 WP_001275017.1 NZ_CP089790:299192-299461 [Salmonella enterica subsp. enterica serovar Kentucky]
MSALKKQRIDLRLTDGDKSMIEEAAAITNQSITQFMLNSAAERAAEVLEQHRRVILNEASWARVMDALSHPPTPNEKLKR
AAQRLQDME

Nucleotide

• Length: 270 bp

>AT229308 NZ_CP124436:3484406-3484657 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A5H5BA59

Antitoxin

• AlphaFold DB: A0A5I8URP5