Detailed information of TA system TA022928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3530394..3531025
• Replicon: chromosome
• Accession: NC_010842
• Organism: Leptospira biflexa serovar Patoc strain Patoc 1 (Ames)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0SRH1
• Locus tag: LBF_RS16690
• Protein ID: WP_012390319.1
• Coordinates: 3530394..3530798 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0SRH2
• Locus tag: LBF_RS16695
• Protein ID: WP_012390320.1
• Coordinates: 3530795..3531025 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBF_RS16660	3526184..3526459	-	276	WP_012390313.1	thioesterase	-
LBF_RS16665	3526519..3526986	-	468	WP_012390314.1	hypothetical protein	-
LBF_RS16670	3527004..3527339	-	336	WP_012476462.1	OB-fold domain-containing protein	-
LBF_RS16675	3527407..3527787	-	381	WP_012390316.1	nuclear transport factor 2 family protein	-
LBF_RS16680	3527837..3529036	-	1200	WP_012390317.1	SH3 domain-containing protein	-
LBF_RS16685	3529220..3530200	-	981	WP_041770094.1	BtrH N-terminal domain-containing protein	-
LBF_RS16690	3530394..3530798	-	405	WP_012390319.1	type II toxin-antitoxin system VapC family toxin	Toxin
LBF_RS16695	3530795..3531025	-	231	WP_012390320.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LBF_RS16700	3531408..3532622	-	1215	WP_012390321.1	acetyl-CoA C-acetyltransferase	-
LBF_RS16705	3532694..3533767	-	1074	WP_012390322.1	hypothetical protein	-
LBF_RS16710	3533815..3534342	-	528	WP_012390323.1	NADH-quinone oxidoreductase subunit I	-
LBF_RS16715	3534329..3535777	-	1449	WP_012390324.1	(2Fe-2S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15507.87 Da
• Isoelectric Point: 8.7364

>T22928 WP_012390319.1 NC_010842:c3530798-3530394 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Ames)']
MNHYLLDTNICIYIINKKPENVYQKFKKISLDNIFISSITEFELRYGVQKSQKADKNQKTLNEFLGYLNVINFDSESASV
AGTIRTKLEKKGEIIGPYDLLIASQAIASDIILVTNNEKEYKRIKELKIENWIN

Nucleotide

• Length: 405 bp

>T22928 NC_010842:c3530798-3530394 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Ames)']
ATGAATCATTATTTATTAGATACAAATATTTGTATTTATATCATTAATAAAAAACCTGAAAATGTTTACCAAAAATTCAA
AAAAATTAGTTTAGATAACATCTTCATTTCTAGCATCACTGAATTTGAATTAAGATATGGTGTTCAAAAAAGTCAAAAAG
CTGATAAAAATCAAAAAACTCTAAATGAATTTCTGGGTTACCTAAATGTCATAAACTTTGACTCAGAATCAGCATCGGTA
GCAGGAACTATCAGAACTAAACTAGAGAAAAAGGGAGAAATAATTGGACCGTATGATTTACTCATCGCTTCTCAGGCTAT
TGCTAGTGACATTATTTTAGTTACAAATAATGAAAAAGAGTATAAAAGAATTAAAGAACTTAAAATAGAAAACTGGATTA
ATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8935.20 Da
• Isoelectric Point: 10.1006

>AT22928 WP_012390320.1 NC_010842:c3531025-3530795 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Ames)']
MNRAKLFKNGDSQAVRLPKEFRFKGKEVYIRKDGNCVIISPIDDAVDRLWKSLNDFSDDFLIERNQPKSFDKRNSI

Nucleotide

• Length: 231 bp

>AT22928 NC_010842:c3531025-3530795 [Leptospira biflexa serovar Patoc strain 'Patoc 1 (Ames)']
ATGAATCGTGCGAAATTATTTAAAAATGGTGATAGTCAAGCGGTTAGACTCCCTAAAGAATTTAGATTTAAAGGAAAAGA
AGTCTACATTAGAAAAGATGGAAATTGCGTTATTATATCACCTATCGACGATGCCGTTGACAGATTATGGAAGTCTCTAA
ATGATTTTTCGGATGATTTTCTCATTGAGAGAAATCAACCTAAGTCTTTCGACAAACGAAATTCTATATGA

Structures

Toxin

• AlphaFold DB: B0SRH1

Antitoxin

• AlphaFold DB: B0SRH2