Detailed information of TA system TA229259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3168998..3169685
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: LVJ76_RS15115
• Protein ID: WP_003414624.1
• Coordinates: 3169242..3169685 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: LVJ76_RS15110
• Protein ID: WP_003414620.1
• Coordinates: 3168998..3169255 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS15090 (LVJ76_15100)	3164318..3165172	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
LVJ76_RS15095 (LVJ76_15105)	3165228..3166391	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ76_RS15100 (LVJ76_15110)	3166408..3167622	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
LVJ76_RS15105 (LVJ76_15115)	3167630..3168871	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
LVJ76_RS15110 (LVJ76_15120)	3168998..3169255	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
LVJ76_RS15115 (LVJ76_15125)	3169242..3169685	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ76_RS15120 (LVJ76_15130)	3169765..3170427	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
LVJ76_RS15125 (LVJ76_15135)	3170523..3170714	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LVJ76_RS15130 (LVJ76_15140)	3171046..3172794	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
LVJ76_RS15135 (LVJ76_15145)	3172890..3173471	+	582	WP_003414644.1	fasciclin domain-containing protein	-
LVJ76_RS15140 (LVJ76_15150)	3173571..3173837	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T229259 WP_003414624.1 NZ_CP089781:3169242-3169685 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T229259 NZ_CP124424:3468458-3468745 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT229259 WP_003414620.1 NZ_CP089781:3168998-3169255 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT229259 NZ_CP124424:3468732-3469034 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0