229257

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3122480..3123084 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ76_RS14885 Protein ID WP_003414492.1
Coordinates 3122480..3122872 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ76_RS14890 Protein ID WP_003414495.1
Coordinates 3122869..3123084 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS14855 (LVJ76_14870) 3117630..3118418 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ76_RS14860 (LVJ76_14875) 3118752..3119297 - 546 WP_003913758.1 DUF1802 family protein -
LVJ76_RS14865 (LVJ76_14880) 3119569..3120453 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ76_RS14870 (LVJ76_14885) 3120456..3121343 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ76_RS14875 (LVJ76_14890) 3121648..3122193 - 546 WP_003899500.1 DUF1802 family protein -
LVJ76_RS14880 (LVJ76_14895) 3122190..3122459 - 270 WP_003414489.1 DUF2277 family protein -
LVJ76_RS14885 (LVJ76_14900) 3122480..3122872 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ76_RS14890 (LVJ76_14905) 3122869..3123084 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ76_RS14895 (LVJ76_14910) 3123131..3123880 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ76_RS14900 (LVJ76_14915) 3123959..3125041 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ76_RS14905 (LVJ76_14920) 3125034..3126344 - 1311 WP_070890835.1 ABC transporter substrate-binding protein -
LVJ76_RS14910 (LVJ76_14925) 3126347..3127174 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LVJ76_RS14915 (LVJ76_14930) 3127171..3128082 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T229257 WP_003414492.1 NZ_CP089781:c3122872-3122480 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T229257 NZ_CP124424:3284956-3285306 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT229257 WP_003414495.1 NZ_CP089781:c3123084-3122869 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT229257 NZ_CP124424:3284711-3284962 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References