Detailed information of TA system TA229255

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3096123..3096693
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: LVJ76_RS14740
• Protein ID: WP_003414166.1
• Coordinates: 3096123..3096479 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: LVJ76_RS14745
• Protein ID: WP_003901465.1
• Coordinates: 3096463..3096693 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS14720 (LVJ76_14735)	3091575..3093263	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
LVJ76_RS14725 (LVJ76_14740)	3093267..3093593	-	327	WP_003414157.1	hypothetical protein	-
LVJ76_RS14730 (LVJ76_14745)	3093766..3094353	+	588	WP_003914429.1	DUF3558 family protein	-
LVJ76_RS14735 (LVJ76_14750)	3094372..3096021	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
LVJ76_RS14740 (LVJ76_14755)	3096123..3096479	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
LVJ76_RS14745 (LVJ76_14760)	3096463..3096693	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
LVJ76_RS14750 (LVJ76_14765)	3096736..3097779	-	1044	WP_023642154.1	DUF2293 domain-containing protein	-
LVJ76_RS14755 (LVJ76_14770)	3097778..3098245	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
LVJ76_RS14760 (LVJ76_14775)	3098421..3098675	-	255	WP_003917684.1	hypothetical protein	-
LVJ76_RS14765 (LVJ76_14780)	3098823..3099227	+	405	WP_009938577.1	hypothetical protein	-
LVJ76_RS14770 (LVJ76_14785)	3099224..3099415	+	192	WP_003414184.1	hypothetical protein	-
LVJ76_RS14775 (LVJ76_14790)	3099632..3099892	+	261	Protein_2916	transposase	-
LVJ76_RS14780 (LVJ76_14795)	3101002..3101259	+	258	WP_003899489.1	hypothetical protein	-
LVJ76_RS14785 (LVJ76_14800)	3101364..3101675	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T229255 WP_003414166.1 NZ_CP089781:c3096479-3096123 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T229255 NZ_CP124424:2853805-2854116 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT229255 WP_003901465.1 NZ_CP089781:c3096693-3096463 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT229255 NZ_CP124424:2854117-2854407 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3