Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3056126..3056805 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag LVJ76_RS14510 Protein ID WP_003414059.1
Coordinates 3056126..3056542 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LVJ76_RS14515 Protein ID WP_003414061.1
Coordinates 3056539..3056805 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS14490 (LVJ76_14500) 3052178..3053080 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LVJ76_RS14495 (LVJ76_14505) 3053149..3053901 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LVJ76_RS14500 (LVJ76_14515) 3054145..3054420 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LVJ76_RS14505 (LVJ76_14520) 3054417..3056039 - 1623 WP_003906897.1 class I SAM-dependent DNA methyltransferase -
LVJ76_RS14510 (LVJ76_14525) 3056126..3056542 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
LVJ76_RS14515 (LVJ76_14530) 3056539..3056805 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ76_RS14520 (LVJ76_14535) 3056831..3057226 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
LVJ76_RS14525 (LVJ76_14540) 3057223..3057492 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ76_RS14530 (LVJ76_14545) 3057502..3058596 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LVJ76_RS14535 (LVJ76_14550) 3058593..3059012 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
LVJ76_RS14540 (LVJ76_14555) 3059011..3059085 + 75 Protein_2869 hypothetical protein -
LVJ76_RS14545 (LVJ76_14560) 3059086..3059565 - 480 WP_003414073.1 dihydrofolate reductase -
LVJ76_RS14550 (LVJ76_14565) 3059636..3060436 - 801 WP_009939104.1 thymidylate synthase -
LVJ76_RS14555 (LVJ76_14570) 3060592..3061329 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T229253 WP_003414059.1 NZ_CP089781:c3056542-3056126 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T229253 NZ_CP124424:c2467433-2467326 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT229253 WP_003414061.1 NZ_CP089781:c3056805-3056539 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT229253 NZ_CP124424:2467490-2467547 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References