Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2855814..2856445 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ76_RS13415 Protein ID WP_003413174.1
Coordinates 2856068..2856445 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ76_RS13410 Protein ID WP_003413167.1
Coordinates 2855814..2856071 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS13380 (LVJ76_13390) 2851728..2852042 + 315 WP_009937839.1 hypothetical protein -
LVJ76_RS13385 (LVJ76_13395) 2852338..2853549 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ76_RS13390 (LVJ76_13400) 2853676..2854335 + 660 WP_014585545.1 LppA family lipoprotein -
LVJ76_RS13395 (LVJ76_13405) 2854332..2854994 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ76_RS13400 (LVJ76_13410) 2854991..2855269 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ76_RS13405 (LVJ76_13415) 2855362..2855775 + 414 WP_003413164.1 PIN domain nuclease -
LVJ76_RS13410 (LVJ76_13420) 2855814..2856071 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ76_RS13415 (LVJ76_13425) 2856068..2856445 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ76_RS13420 (LVJ76_13430) 2856461..2856835 - 375 WP_003413177.1 hypothetical protein -
LVJ76_RS13425 (LVJ76_13435) 2856935..2857330 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ76_RS13430 (LVJ76_13440) 2857327..2857572 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ76_RS13435 (LVJ76_13445) 2857983..2858402 - 420 WP_003413190.1 A24 family peptidase -
LVJ76_RS13440 (LVJ76_13450) 2858414..2859223 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ76_RS13445 (LVJ76_13455) 2859220..2860473 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ76_RS13450 (LVJ76_13460) 2860466..2860978 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T229249 WP_003413174.1 NZ_CP089781:2856068-2856445 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T229249 NZ_CP124424:1830372-1830749 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT229249 WP_003413167.1 NZ_CP089781:2855814-2856071 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT229249 NZ_CP124424:1829915-1830283 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References