Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2218986..2219572 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LVJ76_RS10405 Protein ID WP_003410010.1
Coordinates 2218986..2219330 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ76_RS10410 Protein ID WP_003410014.1
Coordinates 2219324..2219572 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS10370 (LVJ76_10385) 2214692..2215291 + 600 WP_003409989.1 L-lysine exporter -
LVJ76_RS10375 (LVJ76_10390) 2215707..2216135 + 429 WP_003906713.1 cellulose-binding protein -
LVJ76_RS10380 (LVJ76_10395) 2216361..2216900 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ76_RS10385 (LVJ76_10400) 2217420..2217980 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ76_RS10390 (LVJ76_10405) 2217977..2218318 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ76_RS10395 (LVJ76_10410) 2218404..2218661 + 258 WP_003410006.1 hypothetical protein -
LVJ76_RS10400 (LVJ76_10415) 2218562..2218897 - 336 WP_003410009.1 dehydrogenase -
LVJ76_RS10405 (LVJ76_10420) 2218986..2219330 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ76_RS10410 (LVJ76_10425) 2219324..2219572 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ76_RS10415 (LVJ76_10430) 2219672..2221987 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ76_RS10420 (LVJ76_10435) 2221984..2222256 - 273 WP_003410017.1 DUF1490 family protein -
LVJ76_RS10425 (LVJ76_10440) 2222309..2222665 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ76_RS10430 (LVJ76_10445) 2222822..2223589 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T229240 WP_003410010.1 NZ_CP089781:c2219330-2218986 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T229240 NZ_CP124421:c66161-66027 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT229240 WP_003410014.1 NZ_CP089781:c2219572-2219324 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT229240 NZ_CP124421:66206-66266 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References