Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2210094..2210782 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag LVJ76_RS10340 Protein ID WP_003409958.1
Coordinates 2210094..2210513 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ76_RS10345 Protein ID WP_003409968.1
Coordinates 2210522..2210782 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS10320 (LVJ76_10335) 2205589..2206437 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ76_RS10325 (LVJ76_10340) 2206400..2207845 - 1446 WP_003899115.1 APC family permease -
LVJ76_RS10330 (LVJ76_10345) 2208024..2208710 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ76_RS10335 (LVJ76_10350) 2208901..2209869 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ76_RS10340 (LVJ76_10355) 2210094..2210513 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ76_RS10345 (LVJ76_10360) 2210522..2210782 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ76_RS10350 (LVJ76_10365) 2210925..2212601 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
LVJ76_RS10355 (LVJ76_10370) 2212589..2213242 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ76_RS10360 (LVJ76_10375) 2213357..2213587 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ76_RS10365 (LVJ76_10380) 2213672..2214583 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ76_RS10370 (LVJ76_10385) 2214692..2215291 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T229238 WP_003409958.1 NZ_CP089781:c2210513-2210094 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T229238 NZ_CP124420:c4563376-4563158 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT229238 WP_003409968.1 NZ_CP089781:c2210782-2210522 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT229238 NZ_CP124420:c4563776-4563402 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References