Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1760710..1761323 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ76_RS08275 Protein ID WP_003407786.1
Coordinates 1760919..1761323 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ76_RS08270 Protein ID WP_009935474.1
Coordinates 1760710..1760913 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS08240 (LVJ76_08250) 1756115..1756495 + 381 WP_003407768.1 fumarate reductase subunit C -
LVJ76_RS08245 (LVJ76_08255) 1756492..1756869 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ76_RS08250 (LVJ76_08260) 1756937..1757545 + 609 WP_070890827.1 TetR/AcrR family transcriptional regulator -
LVJ76_RS08255 (LVJ76_08265) 1757729..1758877 + 1149 Protein_1628 MMPL family transporter -
LVJ76_RS08260 (LVJ76_08270) 1758887..1759333 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
LVJ76_RS08265 (LVJ76_08275) 1759368..1760657 + 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ76_RS08270 (LVJ76_08280) 1760710..1760913 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ76_RS08275 (LVJ76_08285) 1760919..1761323 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ76_RS08280 (LVJ76_08290) 1761340..1763082 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ76_RS08285 (LVJ76_08295) 1763075..1765372 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T229232 WP_003407786.1 NZ_CP089781:1760919-1761323 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T229232 NZ_CP124420:3716032-3716205 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT229232 WP_009935474.1 NZ_CP089781:1760710-1760913 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT229232 NZ_CP124420:3715708-3716004 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References