Detailed information of TA system TA229226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1236745..1237313
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ76_RS05935
• Protein ID: WP_003405865.1
• Coordinates: 1236939..1237313 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ76_RS05930
• Protein ID: WP_003405863.1
• Coordinates: 1236745..1236942 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS05910 (LVJ76_05915)	1232786..1233424	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ76_RS05915 (LVJ76_05920)	1233514..1234521	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ76_RS05920 (LVJ76_05925)	1234538..1235521	-	984	WP_003898731.1	hypothetical protein	-
LVJ76_RS05925 (LVJ76_05930)	1235584..1236657	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ76_RS05930 (LVJ76_05935)	1236745..1236942	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ76_RS05935 (LVJ76_05940)	1236939..1237313	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ76_RS05940 (LVJ76_05945)	1237516..1238214	+	699	WP_003898733.1	hypothetical protein	-
LVJ76_RS05945 (LVJ76_05950)	1238332..1238517	+	186	WP_003901093.1	hypothetical protein	-
LVJ76_RS05950 (LVJ76_05955)	1238444..1238719	-	276	WP_003405867.1	hypothetical protein	-
LVJ76_RS05955 (LVJ76_05960)	1238962..1239285	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ76_RS05960 (LVJ76_05965)	1239300..1239998	-	699	WP_031646953.1	hypothetical protein	-
LVJ76_RS05965 (LVJ76_05970)	1240032..1240241	+	210	WP_003911400.1	hypothetical protein	-
LVJ76_RS05970 (LVJ76_05975)	1240193..1240963	-	771	Protein_1176	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T229226 WP_003405865.1 NZ_CP089781:1236939-1237313 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T229226 NZ_CP124420:c2534133-2533819 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT229226 WP_003405863.1 NZ_CP089781:1236745-1236942 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT229226 NZ_CP124420:c2533822-2533406 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07