Detailed information of TA system TA229222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1011981..1012600
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LVJ76_RS04805
• Protein ID: WP_003404726.1
• Coordinates: 1012166..1012600 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: LVJ76_RS04800
• Protein ID: WP_003898641.1
• Coordinates: 1011981..1012160 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS04785 (LVJ76_04790)	1007454..1009034	+	1581	WP_003909313.1	serine hydrolase	-
LVJ76_RS04790 (LVJ76_04795)	1009031..1011424	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
LVJ76_RS04795 (LVJ76_04800)	1011697..1011855	+	159	WP_003404720.1	hypothetical protein	-
LVJ76_RS04800 (LVJ76_04805)	1011981..1012160	+	180	WP_003898641.1	antitoxin	Antitoxin
LVJ76_RS04805 (LVJ76_04810)	1012166..1012600	+	435	WP_003404726.1	SRPBCC family protein	Toxin
LVJ76_RS04810 (LVJ76_04815)	1012698..1013471	+	774	WP_003404735.1	VOC family protein	-
LVJ76_RS04815 (LVJ76_04820)	1013536..1013985	+	450	WP_003404738.1	hypothetical protein	-
LVJ76_RS04820 (LVJ76_04825)	1014120..1014350	-	231	WP_003898642.1	hypothetical protein	-
LVJ76_RS04825 (LVJ76_04830)	1014517..1016025	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
LVJ76_RS04830 (LVJ76_04835)	1016027..1017265	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T229222 WP_003404726.1 NZ_CP089781:1012166-1012600 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T229222 NZ_CP124420:c1287569-1287315 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT229222 WP_003898641.1 NZ_CP089781:1011981-1012160 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT229222 NZ_CP124420:c1287817-1287566 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5