Detailed information of TA system TA229212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 693427..694073
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: LVJ76_RS03130
• Protein ID: WP_003403137.1
• Coordinates: 693427..693840 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ76_RS03135
• Protein ID: WP_003403139.1
• Coordinates: 693837..694073 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS03110 (LVJ76_03115)	689510..691060	+	1551	WP_003403119.1	MlaD family protein	-
LVJ76_RS03115 (LVJ76_03120)	691112..691504	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ76_RS03120 (LVJ76_03125)	691501..691758	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ76_RS03125 (LVJ76_03130)	691941..693176	-	1236	WP_003403128.1	ATP-binding protein	-
LVJ76_RS03130 (LVJ76_03135)	693427..693840	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ76_RS03135 (LVJ76_03140)	693837..694073	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ76_RS03140 (LVJ76_03145)	694177..694683	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ76_RS03145 (LVJ76_03150)	694797..695327	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ76_RS03150 (LVJ76_03155)	695311..696006	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ76_RS03155 (LVJ76_03160)	696129..696440	+	312	WP_003403164.1	hypothetical protein	-
LVJ76_RS03160 (LVJ76_03165)	696512..697462	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ76_RS03165 (LVJ76_03170)	697703..698287	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ76_RS03170 (LVJ76_03175)	698289..698999	+	711	Protein_628	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T229212 WP_003403137.1 NZ_CP089781:c693840-693427 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T229212 NZ_CP124415:4078551-4078853 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT229212 WP_003403139.1 NZ_CP089781:c694073-693837 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT229212 NZ_CP124415:4078889-4079230 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89