Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 691112..691758 Replicon chromosome
Accession NZ_CP089781
Organism Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LVJ76_RS03115 Protein ID WP_003403122.1
Coordinates 691112..691504 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LVJ76_RS03120 Protein ID WP_003403125.1
Coordinates 691501..691758 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ76_RS03100 (LVJ76_03105) 686774..688300 + 1527 WP_003403112.1 virulence factor Mce family protein -
LVJ76_RS03105 (LVJ76_03110) 688363..689505 + 1143 WP_003911253.1 virulence factor Mce family protein -
LVJ76_RS03110 (LVJ76_03115) 689510..691060 + 1551 WP_003403119.1 MlaD family protein -
LVJ76_RS03115 (LVJ76_03120) 691112..691504 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LVJ76_RS03120 (LVJ76_03125) 691501..691758 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LVJ76_RS03125 (LVJ76_03130) 691941..693176 - 1236 WP_003403128.1 ATP-binding protein -
LVJ76_RS03130 (LVJ76_03135) 693427..693840 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
LVJ76_RS03135 (LVJ76_03140) 693837..694073 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LVJ76_RS03140 (LVJ76_03145) 694177..694683 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ76_RS03145 (LVJ76_03150) 694797..695327 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LVJ76_RS03150 (LVJ76_03155) 695311..696006 - 696 WP_003918099.1 two-component system response regulator TcrA -
LVJ76_RS03155 (LVJ76_03160) 696129..696440 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T229211 WP_003403122.1 NZ_CP089781:c691504-691112 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T229211 NZ_CP124415:c4049597-4049379 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT229211 WP_003403125.1 NZ_CP089781:c691758-691501 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT229211 NZ_CP124415:c4049997-4049623 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References