Detailed information of TA system TA229198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3172716..3173403
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: LVJ75_RS15135
• Protein ID: WP_003414624.1
• Coordinates: 3172960..3173403 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: LVJ75_RS15130
• Protein ID: WP_003414620.1
• Coordinates: 3172716..3172973 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS15110 (LVJ75_15095)	3168036..3168890	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
LVJ75_RS15115 (LVJ75_15100)	3168946..3170109	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
LVJ75_RS15120 (LVJ75_15105)	3170126..3171340	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
LVJ75_RS15125 (LVJ75_15110)	3171348..3172589	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
LVJ75_RS15130 (LVJ75_15115)	3172716..3172973	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
LVJ75_RS15135 (LVJ75_15120)	3172960..3173403	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS15140 (LVJ75_15125)	3173483..3174145	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
LVJ75_RS15145 (LVJ75_15130)	3174241..3174432	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
LVJ75_RS15150 (LVJ75_15135)	3174764..3176512	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
LVJ75_RS15155 (LVJ75_15140)	3176608..3177189	+	582	WP_003414644.1	fasciclin domain-containing protein	-
LVJ75_RS15160 (LVJ75_15145)	3177289..3177555	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T229198 WP_003414624.1 NZ_CP089780:3172960-3173403 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T229198 NZ_CP124415:1860818-1861195 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT229198 WP_003414620.1 NZ_CP089780:3172716-3172973 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT229198 NZ_CP124415:1860361-1860729 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0