Detailed information of TA system TA229193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3066415..3067102
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: LVJ75_RS14560
• Protein ID: WP_003414064.1
• Coordinates: 3066707..3067102 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LVJ75_RS14555
• Protein ID: WP_003414061.1
• Coordinates: 3066415..3066681 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS14530 (LVJ75_14515)	3062054..3062956	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LVJ75_RS14535 (LVJ75_14520)	3063025..3063777	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LVJ75_RS14540 (LVJ75_14530)	3064021..3064296	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LVJ75_RS14545 (LVJ75_14535)	3064293..3065915	-	1623	WP_003414057.1	class I SAM-dependent DNA methyltransferase	-
LVJ75_RS14550 (LVJ75_14540)	3066002..3066418	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
LVJ75_RS14555 (LVJ75_14545)	3066415..3066681	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ75_RS14560 (LVJ75_14550)	3066707..3067102	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS14565 (LVJ75_14555)	3067099..3067368	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ75_RS14570 (LVJ75_14560)	3067378..3068472	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
LVJ75_RS14575 (LVJ75_14565)	3068469..3068888	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LVJ75_RS14580 (LVJ75_14570)	3068887..3068961	+	75	Protein_2877	hypothetical protein	-
LVJ75_RS14585 (LVJ75_14575)	3068962..3069441	-	480	WP_003414073.1	dihydrofolate reductase	-
LVJ75_RS14590 (LVJ75_14580)	3069512..3070312	-	801	WP_003911953.1	thymidylate synthase	-
LVJ75_RS14595 (LVJ75_14585)	3070468..3071205	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T229193 WP_003414064.1 NZ_CP089780:c3067102-3066707 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T229193 NZ_CP124415:c519987-519885 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT229193 WP_003414061.1 NZ_CP089780:c3066681-3066415 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT229193 NZ_CP124415:519847-520115 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64