Detailed information of TA system TA229190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2927012..2927726
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: LVJ75_RS13730
• Protein ID: WP_003413460.1
• Coordinates: 2927286..2927726 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: LVJ75_RS13725
• Protein ID: WP_003413456.1
• Coordinates: 2927012..2927299 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS13690 (LVJ75_13675)	2922434..2922679	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ75_RS13695 (LVJ75_13680)	2922676..2923080	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
LVJ75_RS13700 (LVJ75_13685)	2923297..2923917	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
LVJ75_RS13705 (LVJ75_13690)	2923928..2924422	+	495	WP_003413444.1	DUF2617 family protein	-
LVJ75_RS13710 (LVJ75_13695)	2924419..2924850	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
LVJ75_RS13715 (LVJ75_13700)	2924875..2925333	+	459	WP_003413451.1	DUF350 domain-containing protein	-
LVJ75_RS13720 (LVJ75_13705)	2925330..2926901	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
LVJ75_RS13725 (LVJ75_13710)	2927012..2927299	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
LVJ75_RS13730 (LVJ75_13715)	2927286..2927726	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS13735 (LVJ75_13720)	2927747..2928502	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LVJ75_RS13740 (LVJ75_13725)	2928635..2929231	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
LVJ75_RS13745 (LVJ75_13730)	2929239..2930084	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
LVJ75_RS13750 (LVJ75_13735)	2930113..2931012	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
LVJ75_RS13755 (LVJ75_13740)	2931140..2931814	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T229190 WP_003413460.1 NZ_CP089780:2927286-2927726 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T229190 NZ_CP124411:c116282-115977 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT229190 WP_003413456.1 NZ_CP089780:2927012-2927299 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT229190 NZ_CP124411:c116502-116284 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8