229189

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2866670..2867307 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ75_RS13465 Protein ID WP_003413180.1
Coordinates 2866670..2867065 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ75_RS13470 Protein ID WP_003413183.1
Coordinates 2867062..2867307 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS13425 (LVJ75_13410) 2862074..2863285 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ75_RS13430 (LVJ75_13415) 2863412..2864071 + 660 WP_003902296.1 LppA family lipoprotein -
LVJ75_RS13435 (LVJ75_13420) 2864068..2864730 + 663 WP_003905878.1 LppA family lipoprotein -
LVJ75_RS13440 (LVJ75_13425) 2864727..2865004 + 278 Protein_2653 type II toxin-antitoxin system VapB family antitoxin -
LVJ75_RS13445 (LVJ75_13430) 2865097..2865510 + 414 WP_003413164.1 PIN domain nuclease -
LVJ75_RS13450 (LVJ75_13435) 2865549..2865806 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ75_RS13455 (LVJ75_13440) 2865803..2866180 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ75_RS13460 (LVJ75_13445) 2866196..2866570 - 375 WP_003413177.1 hypothetical protein -
LVJ75_RS13465 (LVJ75_13450) 2866670..2867065 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ75_RS13470 (LVJ75_13455) 2867062..2867307 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ75_RS13475 (LVJ75_13460) 2867718..2868137 - 420 WP_003413190.1 A24 family peptidase -
LVJ75_RS13480 (LVJ75_13465) 2868149..2868958 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ75_RS13485 (LVJ75_13470) 2868955..2870208 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ75_RS13490 (LVJ75_13475) 2870201..2870713 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T229189 WP_003413180.1 NZ_CP089780:c2867065-2866670 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T229189 NZ_CP124411:c67912-67778 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT229189 WP_003413183.1 NZ_CP089780:c2867307-2867062 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT229189 NZ_CP124411:67957-68017 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References