229188

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2865549..2866180 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ75_RS13455 Protein ID WP_003413174.1
Coordinates 2865803..2866180 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ75_RS13450 Protein ID WP_003413167.1
Coordinates 2865549..2865806 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS13420 (LVJ75_13405) 2861464..2861778 + 315 WP_009937839.1 hypothetical protein -
LVJ75_RS13425 (LVJ75_13410) 2862074..2863285 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ75_RS13430 (LVJ75_13415) 2863412..2864071 + 660 WP_003902296.1 LppA family lipoprotein -
LVJ75_RS13435 (LVJ75_13420) 2864068..2864730 + 663 WP_003905878.1 LppA family lipoprotein -
LVJ75_RS13440 (LVJ75_13425) 2864727..2865004 + 278 Protein_2653 type II toxin-antitoxin system VapB family antitoxin -
LVJ75_RS13445 (LVJ75_13430) 2865097..2865510 + 414 WP_003413164.1 PIN domain nuclease -
LVJ75_RS13450 (LVJ75_13435) 2865549..2865806 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ75_RS13455 (LVJ75_13440) 2865803..2866180 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ75_RS13460 (LVJ75_13445) 2866196..2866570 - 375 WP_003413177.1 hypothetical protein -
LVJ75_RS13465 (LVJ75_13450) 2866670..2867065 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ75_RS13470 (LVJ75_13455) 2867062..2867307 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ75_RS13475 (LVJ75_13460) 2867718..2868137 - 420 WP_003413190.1 A24 family peptidase -
LVJ75_RS13480 (LVJ75_13465) 2868149..2868958 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ75_RS13485 (LVJ75_13470) 2868955..2870208 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ75_RS13490 (LVJ75_13475) 2870201..2870713 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T229188 WP_003413174.1 NZ_CP089780:2865803-2866180 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T229188 NZ_CP124410:c4918686-4918579 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT229188 WP_003413167.1 NZ_CP089780:2865549-2865806 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT229188 NZ_CP124410:4918734-4918800 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References