Detailed information of TA system TA229186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2851219..2851859
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ75_RS13360
• Protein ID: WP_003412970.1
• Coordinates: 2851219..2851638 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ75_RS13365
• Protein ID: WP_003412975.1
• Coordinates: 2851635..2851859 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS13330 (LVJ75_13315)	2846809..2847531	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ75_RS13335 (LVJ75_13320)	2848048..2848270	+	223	Protein_2632	type II toxin-antitoxin system VapB family antitoxin	-
LVJ75_RS13340 (LVJ75_13325)	2848267..2848668	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ75_RS13345 (LVJ75_13330)	2848703..2849623	-	921	WP_003412965.1	restriction endonuclease	-
LVJ75_RS13350 (LVJ75_13335)	2849964..2850209	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ75_RS13355 (LVJ75_13340)	2850268..2851218	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ75_RS13360 (LVJ75_13345)	2851219..2851638	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ75_RS13365 (LVJ75_13350)	2851635..2851859	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ75_RS13370 (LVJ75_13355)	2851890..2854725	-	2836	Protein_2639	amino acid decarboxylase	-
LVJ75_RS13375 (LVJ75_13360)	2854797..2855198	-	402	WP_003412981.1	hypothetical protein	-
LVJ75_RS13380 (LVJ75_13365)	2855198..2855668	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ75_RS13385 (LVJ75_13370)	2855671..2856234	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T229186 WP_003412970.1 NZ_CP089780:c2851638-2851219 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T229186 NZ_CP124410:c4074988-4074770 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT229186 WP_003412975.1 NZ_CP089780:c2851859-2851635 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT229186 NZ_CP124410:c4075388-4075014 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09