Detailed information of TA system TA229184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2402477..2403006
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: LVJ75_RS11265
• Protein ID: WP_003411124.1
• Coordinates: 2402477..2402794 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: LVJ75_RS11270
• Protein ID: WP_003411127.1
• Coordinates: 2402791..2403006 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS11235 (LVJ75_11230)	2397498..2398574	+	1077	WP_003411110.1	hypothetical protein	-
LVJ75_RS11240 (LVJ75_11235)	2398571..2398852	+	282	WP_003411112.1	DUF5703 family protein	-
LVJ75_RS11245 (LVJ75_11240)	2398888..2399961	+	1074	WP_003901338.1	quinone-dependent dihydroorotate dehydrogenase	-
LVJ75_RS11250 (LVJ75_11245)	2399966..2400496	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
LVJ75_RS11255 (LVJ75_11250)	2400544..2401890	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
LVJ75_RS11265 (LVJ75_11260)	2402477..2402794	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ75_RS11270 (LVJ75_11265)	2402791..2403006	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
LVJ75_RS11275 (LVJ75_11270)	2403261..2404319	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
LVJ75_RS11280 (LVJ75_11275)	2404449..2404805	-	357	WP_003411130.1	hypothetical protein	-
LVJ75_RS11285 (LVJ75_11280)	2404900..2405682	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
LVJ75_RS11290 (LVJ75_11285)	2405950..2406240	-	291	WP_003900476.1	YggT family protein	-
LVJ75_RS11295 (LVJ75_11290)	2406402..2407058	-	657	WP_003411133.1	cell division protein SepF	-
LVJ75_RS11300 (LVJ75_11295)	2407120..2407899	-	780	WP_015575160.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T229184 WP_003411124.1 NZ_CP089780:c2402794-2402477 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T229184 NZ_CP124410:3622662-3622946 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT229184 WP_003411127.1 NZ_CP089780:c2403006-2402791 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT229184 NZ_CP124410:3622426-3622659 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4