Detailed information of TA system TA229180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2250364..2250990
• Replicon: chromosome
• Accession: NZ_CP089780
• Organism: Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: LVJ75_RS10545
• Protein ID: WP_003410075.1
• Coordinates: 2250592..2250990 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: LVJ75_RS10540
• Protein ID: WP_003911750.1
• Coordinates: 2250364..2250591 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ75_RS10530 (LVJ75_10525)	2248403..2248747	-	345	WP_003410065.1	ferredoxin family protein	-
LVJ75_RS10535 (LVJ75_10530)	2248936..2250105	-	1170	WP_003899126.1	ATP-binding protein	-
LVJ75_RS10540 (LVJ75_10535)	2250364..2250591	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ75_RS10545 (LVJ75_10540)	2250592..2250990	+	399	WP_003410075.1	PIN domain nuclease	Toxin
LVJ75_RS10550 (LVJ75_10545)	2251173..2251604	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
LVJ75_RS10555 (LVJ75_10550)	2251705..2252139	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
LVJ75_RS10560 (LVJ75_10555)	2252576..2252755	-	180	Protein_2087	hypothetical protein	-
LVJ75_RS10565 (LVJ75_10560)	2252843..2253463	+	621	WP_003410086.1	IS110 family transposase	-
LVJ75_RS10570 (LVJ75_10565)	2253417..2254007	+	591	WP_003899131.1	IS110 family transposase	-
LVJ75_RS10575 (LVJ75_10570)	2254135..2255391	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
LVJ75_RS10580 (LVJ75_10575)	2255745..2255945	+	201	Protein_2091	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T229180 WP_003410075.1 NZ_CP089780:2250592-2250990 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T229180 NZ_CP089780:2250592-2250990 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT229180 WP_003911750.1 NZ_CP089780:2250364-2250591 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT229180 NZ_CP089780:2250364-2250591 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8