229179

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2226625..2227211 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LVJ75_RS10440 Protein ID WP_003410010.1
Coordinates 2226625..2226969 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ75_RS10445 Protein ID WP_003410014.1
Coordinates 2226963..2227211 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS10405 (LVJ75_10400) 2222331..2222930 + 600 WP_003910883.1 L-lysine exporter -
LVJ75_RS10410 (LVJ75_10405) 2223346..2223774 + 429 WP_003409992.1 cellulose-binding protein -
LVJ75_RS10415 (LVJ75_10410) 2224000..2224539 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ75_RS10420 (LVJ75_10415) 2225059..2225619 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ75_RS10425 (LVJ75_10420) 2225616..2225957 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ75_RS10430 (LVJ75_10425) 2226043..2226300 + 258 WP_003410006.1 hypothetical protein -
LVJ75_RS10435 (LVJ75_10430) 2226201..2226536 - 336 WP_003410009.1 dehydrogenase -
LVJ75_RS10440 (LVJ75_10435) 2226625..2226969 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ75_RS10445 (LVJ75_10440) 2226963..2227211 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ75_RS10450 (LVJ75_10445) 2227311..2229626 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ75_RS10455 (LVJ75_10450) 2229623..2229895 - 273 WP_003410017.1 DUF1490 family protein -
LVJ75_RS10460 (LVJ75_10455) 2229948..2230304 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ75_RS10465 (LVJ75_10460) 2230461..2231228 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T229179 WP_003410010.1 NZ_CP089780:c2226969-2226625 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T229179 NZ_CP124410:2899331-2899642 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT229179 WP_003410014.1 NZ_CP089780:c2227211-2226963 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT229179 NZ_CP124410:2899643-2899933 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References